One-Photon Fluorescence Imaging Essays

Introduction Two-photon excitation is the technique by which a fluorophore is stimulated by the uptake of two photons of minimum energy and greater spectrum compared to the single photon used for traditional excitation. This is the basis for two-photon microscopy. In 1990, a method called two-photon microscopy was developed for capturing high-resolution, three-dimensional images of ... Read More